Senior Application Engineer, Beckman Coulter Life Sciences
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Webinar: Looking under the Hood and Getting Started with the Optima AUC
October 1, 2019 @ 11:00 am - 12:00 pm EDT
ABSTRACT:
DATE: Tuesday, October 1, 2019
TIME: 11AM-12PM, EST
In the previous webinar, Introduction to Characterization of Biomolecules Using Analytical Ultracentrifugation, we discussed the fundamentals of analytical ultracentrifugation and looked at a few sample applications. In this webinar, we explore the operational aspects of this biophysical technique. We will start off with an overview of the technology behind AUC, taking a close look at the two types of detection apparatus offered on the current generation Optima AUC: absorbance and interference. We also look at the improvements offered by the Optima AUC over the previous generation ProteomeLab. Then, we go through some of the steps involved in choosing suitable parameters when planning an experiment on a new sample – including the use of simulation tools available in analysis software packages. We also look at the effect of rotor speed on simulated scan data for two antibody fragments. We learn how to identify incomplete sedimentation, lack of separation between two analytes, and the tradeoffs between rotor speed, number of scans, and experiment duration. We then study improvements in data resolution with increasing rotor speed using simulated data for a typical gene therapy analyte. Finally, we move to instrument control and look at the steps involved in setting up a new experiment, viewing live scans, and transferring data after experiment completion.
Learning Objectives:
- Learn the technical advancements of the Optima AUC and the difference between absorbance and interference mode detection.
- Learn how to design a new experiment in the Optima AUC.
- Learn how to transfer data after an experiment.
- Learn how to use available simulation tools to model scans for a two-analyte system.
Speakers:
Akash Bhattacharya, PhD
Akash Bhattacharya graduated from Presidency College, India with a major in Physics and went on to a Masters’ in Physics at the Indian Institute of Science where he worked on Quantum Computing. He then moved for doctoral studies in Biophysics to the University of Michigan, Ann Arbor where he joined the lab of Prof. Erik Zuiderweg and worked on NMR spectroscopy methods development in the context of the Structural Biology of Chaperone Proteins. After Michigan, he worked briefly at Rutgers and then eventually moved to the Dept. of Biochemistry and Structural Biology at the University of Texas Health at San Antonio. Here, he worked with Prof. Dmitri Ivanov and Prof. Borries Demeler on the enzymology of HIV infection and restriction by mammalian proteins. He also worked on projects related to oncology (DNA damage repair) and neuroscience (voltage gated ion channels), using a wide variety of techniques ranging from X-ray crystallography, NMR spectroscopy, fluorescence spectroscopy, molecular dynamics and analytical ultracentrifugation (AUC). He collaborated with Prof. Demeler to extend AUC methods to novel enzymatic systems resulting in publications in PNAS, Cell Reports, Nature Scientific Reports, etc. Akash joined Beckman Coulter Life Sciences in Oct 2018. He is based in the Colorado R&D center and works on developing new AUC applications. His research interests include extending AUC methodology to new therapeutic areas such as AAV capsids (gene therapy), liposomal drug carriers and others.
Ross VerHeul
Senior Applications Scientist – Beckman Coulter
Ross holds formal training spanning biology to synthetic organic chemistry and wide-ranging expertise in microfluidic applications paired with the production, purification, and characterization of a multitude of biomaterials for use in vitro and in vivo. Such materials include synthetic and natural proteins, polymers, nanoparticles, and viral & non-viral vectors for gene therapy.