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Qiagen: miRNA qPCR for Challenging Samples
MicroRNAs (miRNAs), small non-coding RNAs that regulate gene expression, hold incredible promise as minimally invasive biomarkers for use in the diagnosis and prognosis of a wide range of diseases and biological processes. miRNAs are transported in body fluids within exosomes, thus making them ideal biomarkers due to their stability and the ease with which they can be obtained. However, special challenges exist for miRNA analysis, including the short length of miRNAs, their divergent sequences and variable GC content, as well as a their low abundance in biofluids. Nonetheless, the stable nature of miRNAs makes these short 21-23 nt molecules ideal for use as biomarkers of cancer and disease states. A highly sensitive, specific, and accurate analysis method that allows linear detection of miRNA even at very low concentrations is, therefore, essential for their study.
This webinar discusses the important considerations of miRNA analysis when working with challenging sample types. Use of Locked Nucleic Acid (LNA®) technology is proving instrumental for success in these situations. Discover how LNA® technology, an integral part of the miRCURY LNA miRNA PCR System, deals with challenging clinical biofluid samples and learn about the importance of quality control and data analysis in your PCR experiment.